Generation of double-virus-resistant marker-free transgenic potato plants
نویسندگان
چکیده
منابع مشابه
Generation of Marker-free Transgenic Plants Concurrently Resistant to a DNA Geminivirus and a RNA Tospovirus
Global threats of ssDNA geminivirus and ss(-)RNA tospovirus on crops necessitate the development of transgenic resistance. Here, we constructed a two-T DNA vector carrying a hairpin of the intergenic region (IGR) of Ageratum yellow vein virus (AYVV), residing in an intron inserted in an untranslatable nucleocapsid protein (NP) fragment of Melon yellow spot virus (MYSV). Transgenic tobacco lines...
متن کاملMethods to produce marker-free transgenic plants.
Selectable marker genes (SMGs) have been extraordinarily useful in enabling plant transformation because of the low efficiency of transgene integration. The most used SMGs encode proteins resistant to antibiotics or herbicides and use negative selection, i.e., by killing nontransgenic tissue. However, there are perceived risks in wide-scale deployment of SMG-transgenic plants, and therefore res...
متن کاملEvaluation of Substantial Equivalence of salinity-resistant transgenic potato
Transgenic plants and their products are being developed day by day due to their improved characteristics, and it is necessary to evaluate the safety of these plants before releasing them. Hence, the importance of the issue of biosafety of transgenic plants and the use of their products has led the regulatory agencies to create some laws called substantial equivalence. based on that, the essent...
متن کاملGeneration of Marker- and/or Backbone-Free Transgenic Wheat Plants via Agrobacterium-Mediated Transformation
Horizontal transfer of antibiotic resistance genes to animals and vertical transfer of herbicide resistance genes to the weedy relatives are perceived as major biosafety concerns in genetically modified (GM) crops. In this study, five novel vectors which used gusA and bar as a reporter gene and a selection marker gene, respectively, were constructed based on the pCLEAN dual binary vector system...
متن کاملEfficient generation of marker-free transgenic rice plants using an improved transposon-mediated transgene reintegration strategy.
Marker-free transgenic plants can be developed through transposon-mediated transgene reintegration, which allows intact transgene insertion with defined boundaries and requires only a few primary transformants. In this study, we improved the selection strategy and validated that the maize (Zea mays) Activator/Dissociation (Ds) transposable element can be routinely used to generate marker-free t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Progress in Natural Science
سال: 2009
ISSN: 1002-0071
DOI: 10.1016/j.pnsc.2008.08.005